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Murashige and Skoog (MS) Modified Multiplication Medium C GamborgContains Macro and Micronutrients as described by Murashige & Skoog (1962) and the Vitamins as described by Linsmaier and Skoog (1965) modified with addition of Kinetin, Adenine Hemisulfate, Sodium Phosphate Monobasic, NAA, and Sucrose Cytokines and Auxins should be added as needed Provided as 1 unit to make 10L, 25L, or 50L of medium
Diluting Amphotericin B Solution 100X to 1X concentration is recommended to avoid toxicity in eukaryotic cells in culture
44 g/L in distilled water before use in plant tissue culture
Affects the biosynthesis of branched chain amino acids
Ammonium phosphate
They may be autoclaved as desired and reused indefinitely
Murashige and Skoog Basal Medium should be dissolved at 4
See 'Carbenicillin resistance of Pseudomonas aeruginosa' by PubMed
Aids in the detection of microbial contaminants
or 50L of medium
bioWORLD carries a pure Macerozyme R-10 as well as a Cellulase Enzyme Solution
Powder: Store below 30ºC in the dark
25mM PotassiumPhosphate (KPO4) pH 6
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